Marignani
Discovery Research
Laboratory
Pipeline to precision health
About Our Science
LKB1 Tumour Suppressor Kinase
LKB1s first interacting partners was identified by Dr. Marignani while a post-doctoral fellow at Harvard. Using in vitro expression cloning (IVEC) strategy she developed along with another PDF, LKB1 was discovered to bind to the SWI-SNF family member SMARCA4 (BRG1).
Neurodegenerative Diseases
The human brain has one of the highest metabolic rates in the body, consuming approximately 20% of the energy produced by the body. Changes in brain metabolism occur throughout the aging process in healthy brains and in neurodegenerative diseases, including Alzheimer’s disease (AD).
Single-Cell Genomic Analysis
Our lab uses scRNA-seq, scATAC-seq and snRNA-seq for the purpose of identifying new markers for the various cancers and neurodegenerative diseases.
Cytoskeleton
Our goal is to characterize these new interacting partners in an effort to better understand how VAV2 is involved in cytoskeletal reorganization that leads to cell migration.
Core Activities
The Marignani Discovery Research Laboratory is interested in molecular mechanisms of...
Breast, lung and pancreatic cancers
neurodegenerative diseases
molecular processes that permit cells to migrate
Platforms
single-cell RNA-seq (scRNA-seq)
scATAC-seq
proteomics
metabolomics
epigenomics
immunobiology
whole genome manipulation
gene-editing (CRISPR-Cas9 and shRNA)
transgenic and Cas9 mouse models of human diseases
cell imaging
protein, DNA, and virus manipulations
high through-put screening strategies
- Pre-clinical trials, metronomic dosing, small animal imaging
- Innovation through technology development and refining
- Dissemination of new knowledge: peer-reviewed publications, invited plenary speaker, academic seminar speaker, public outreach
- Mentoring and training of future scientist and medical doctors. Leadership and career development
- Education of stakeholders: people with diseases, their families and friends, community and policy makers
- Resource acquisition and management
Sharing our research with the world
See our work and members in various media engagements, news stories, and reports.
